Sequencing Core
Broad Stem Cell Research Center (BSCRC)
Overview
The High-Throughput Sequencing Core provides the UCLA research community with access to next-generation DNA sequencing technology.
The technology in this Core offers capabilities including SNP mapping, RNA expression measurements, ChIP-seq and DNA methylation analysis at a genome-wide scale.
Such capabilities enable researchers to study the regulation of thousands of genes in a single experiment, providing critical insights into the molecular biology of a cell.
The Core is located in the Terasaki Life Sciences Building, Room 3110.
Leadership
Contact
Website: Sequencing Core
Equipment
Sequencers
Illumina NovaSeq X Plus
State-of-the-art next-generation sequencing (NGS) system that offers unparalleled accuracy, speed and throughput at the lowest possible cost for RNA-seq, whole genome, whole exome and targeted DNA sequencing.
Illumina iSeq
A flexible sequencing system with a wide range of applications that is ideal for small genome sequencing, targeted and amplicon sequencing as well as viral and microbial sequencing.
QC Equipment
Perkin Elmer Janus G3 liquid handler
A library preparation device programmed to execute several protocols for DNA-seq (whole genome, ChIP) and RNA-seq library preparation.
Agilent TapeStation 4200
A quality control system used for automated electrophoresis of 1 to 96 samples. It is strongly recommended that this platform be used for all quality control steps of next-generation sequencing experiments.
Pricing
| "Service Description" | Read Number | Internal Rate | External Rate Non-Profits | External Rate |
|---|---|---|---|---|
| iSeq 100 2x150bp | 4 million | $716 | $806 | $838 |
| NovaSeq X Plus - 1.5B 2x50bp | 800 million | $2,046 | $2,433 | $2,846 |
| NovaSeq X Plus - 1.5B 1x100bp | 800 million | $2,046 | $2,433 | $2,846 |
| NovaSeq X Plus - 1.5B 2x100bp | 800 million | $2,542 | $3,014 | $3,518 |
| NovaSeq X Plus - 1.5B 2x150bp | 800 million | $2,784 | $3,307 | $3,866 |
| NovaSeq X Plus - 10B 2x50bp | 1.25 billion | $1,556 | $1,798 | $2,057 |
| NovaSeq X Plus - 10B 1x100bp | 1.25 billion | $1,556 | $1,798 | $2,057 |
| NovaSeq X Plus - 10B 2x100bp | 1.25 billion | $1,850 | $2,109 | $2,386 |
| NovaSeq X Plus - 10B 2x150bp | 1.25 billion | $1,962 | $2,234 | $2,524 |
| NovaSeq X Plus - 25B 2x150bp | 3.25 billion | $3,168 | $3,538 | $3,933 |