Lipidomics Lab
Overview
The UCLA Lipidomics Lab was founded in 2017. The core has expanded over the last several years in operation, acquiring a second instrument and is in the process of moving to a new dedicated lab space. We analyze several thousand samples each year from UCLA, National and International Clientele. Our flagship lipidomics assay has expanded to become one of the most comprehensive targeted lipidomics assays in use today. We’ve developed our own data analysis application (SLA) that is being utilized by lipidomics labs across North America and Europe. Cumulatively, the core staff have decades of lipid biology experience.
Leadership
Services
Shotgun Lipidomics
This is a direct infusion-tandem mass spectrometry method utilizing a differential mobility system. This assay is performed on the Sciex 5500 Triple-Quadrupole (QQQ) and Sciex 6500+ Triple-Quadrupple (QQQ) with a Shimadzu auto-sampler configured for direct infusion experiments, SelexION ion mobility device, and Shimadzu LC. This assay is built on the Sciex Lipidyzer Platform. We use our in-house data analysis workflow and a combination of 74 lipid standards sourced from Avanti Polar Lipids to provide accurate, quantitative measurements of over 1400 lipid species across 17 lipid subclasses within a biological sample.
Lipid Compositional Analysis
This assay quantitatively measures the fatty acid and cholesterol composition of a biological sample. In situ derivatization breaks up complex esterified lipids into constituent fatty acids and cholesterol, which can be measured by gas chromatography/mass spectrometer. The assay is performed on an Agilent 7890B/5977A GC-MS, and is compatible with stable isotope labeling. Data analysis utilizes the mathematical modeling-based isotopic spectral analysis (ISA) method to provide estimations of flux and lipid synthesis rates, as well as calculating the contribution of carbon sources to the acetyl-CoA pool.
Rates
| Service | Unit | UC Rate | External Rate |
|---|---|---|---|
| Shotgun Lipidomics | per sample | $110 | $175 |
| Shotgun Lipidomics (Bulk 20+) | per sample | $75 | N/A |
| Shotgun Lipidomics with Cholesterol | per sample | $130 | $200 |
| Shotgun Lipidomics with Cholesterol (Bulk 20+) | per sample | $95 | N/A |
| Fatty Acid/Cholesterol Analysis | per hour | $46 | $71 |
| Consultation/Data Analysis | per hour | $100 | $150 |